Inactivation and stability of viral diagnostic reagents treated by gamma radiation
نویسندگان
چکیده
منابع مشابه
Inactivation of rabies diagnostic reagents by gamma radiation.
Treatment of CVS-11 rabies adsorbing suspensions and street rabies infected mouse brains with gamma radiation resulted in inactivated reagents that are safer to distribute and use. These irradiated reagents were as sensitive and reactive as the nonirradiated control reagents.
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Decimal reduction values (D value) for 30 viruses were determined. The weighted D values of the viruses suspended in Eagle's minimum essential medium ranged from 0.39 to 0.53 Mrads. It was necessary to increase the radiation dose by a factor of >3 to inactivate virus suspended in Eagle's minimum essential medium as compared to the same virus suspended in distilled water. The destruction rate cu...
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The Gilchrist and M-33 strains of rubella virus exposed in the frozen state to (137)Ce or (60)Co were inactivated exponentially according to "one hit" kinetics. There was no difference in the radiosensitivity of the two strains. Experimental D(37) values for both strains ranged from 1.9 x 10(5) to 2.9 x 10(5) rads, and computed radiosensitive molecular weights ranged from 2.6 x 10(6) to 4.0 x 1...
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1,1mg I plus 0,42 mg DL-Phenylalanin-(l-14C) wur den in 15 cm3 Wasser gelöst. 1.22 mg II wurden in 15 cm3 Wasser mit 0,5 cm3 7i/lOO-NaOH in das wasser lösliche Mono-Natriumsalz überführt und 0,42 mg d l Phenylalanin-(l-14C) zugefügt. Versuchsdauer 48 Stun den. Die Isolierung und Reinigung der Isoflavone er folgte wie im vorigen Versuch. Ausbeuten: Versuch 1. 4,8 mg Biochanin-A und 1,88 mg F...
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Exposure of Venezuelan equine encephalomyelitis (VEE) virus (at -70 C) to 6 x 10 r gamma-radiation (Co) resulted in loss of lethality for young adult mice and guinea pigs, and loss of capacity to produce plaques or cytopathic effects in tissue culture. The suckling mouse was more sensitive for detecting live virus in radiated suspensions than was the adult mouse or guinea pig. Live virus was de...
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ژورنال
عنوان ژورنال: Biologicals
سال: 1990
ISSN: 1045-1056
DOI: 10.1016/1045-1056(90)90029-y